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Develop as many cloud use cases as you need to document the complete range of cloud enablement patterns and deployment scenarios that you anticipate including in your cloud strategy and roadmap. Below, we provide some sample cloud scenarios that apply aspects of the cloud use case format we developed above. Of course, for your operational planning and formal strategy development needs, you must develop the details of these cloud use cases to support your needs.

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Yeast uses its stored reserves of carbohydrate in order to fuel the early stages of metabolism when it is pitched into wort, for example, the synthesis of sterols There are two principal reserves: glycogen and trehalose Glycogen is similar in structure to the amylopectin fraction of barley starch Trehalose is a disaccharide comprising two glucoses linked with an -1,1 bond between their reducing carbons The glycogen reserves of yeast build up during fermentation and it is important that they are conserved in the yeast during storage between fermentations Trehalose may feature as more of a protection against the stress of starvation It certainly seems to help the survival of yeast under dehydration conditions employed for the storage and shipping of dried yeast Pure yeast culture was pioneered by Hansen at Carlsberg in 1883.

Figure 4 _ 1

That is, H ( G D*y) = 0, which agrees with (29.16). Note that the equations (29.17) re-confirm our earlier remark in (29.6) that the residual vector, y - HG, satisfies the orthogonality condition

By a process of dilution, he was able to isolate individual strains and open up the possibility of selecting and growing separate strains for speci c purposes Nowadays brewers maintain their own pure yeast strains While it is still a fact that some brewers simply use the yeast grown in one fermentation to pitch the following fermentation, and that they have done this for many tens of years, it is much more usual for yeast to be repropagated from a pure culture every 4 6 generations (When brewers talk of generations , they mean successive fermentations; strictly speaking, yeast advances a generation every time it buds, and therefore there are several generations during any individual fermentation) Large quantities of yeast are needed to pitch commercial-scale fermentations They need to be generated by successive scale-up growth from the master culture (Fig 224).

3.0 2.5

Since ux and uy are dimensionless, the angular frequencies u and v are also dimensionless (uz is in units of inverse meters, w is in units of meters; l is, of course, wavelength). It is possible to measure Si (ux , uy , uz , n) by scanning an imaging spectrometer through the focal volume of an imaging system. The general problem of estimating the So (ux , uy , uz , n) from such measurements is an inverse problem typical of tomographic analysis. Note that such an estimation process would be quite different from simply scanning through the focal volume and measuring the image eld. Tomographic analysis reconstructs the object density rather than the eld distribution the object produces. In the present case, the forward mapping from the object spectral density to the image is a convolution. In principle, one could estimate So (ux , uy , uz , n) by deconvolution techniques as discussed in Section 8.5 Such techniques are not likely to be effective, however, because the 3D impulse response of Eqn. (6.74) does not have nite support along the longitudinal axis. In view of this challenge, a variety of techniques have been developed to image in three dimensions without directly inverting Eqn. (6.73), including the projection tomography and structured illumination (optical coherence tomography) strategies discussed in this chapter as well as wavefront coding and radiometric strategies discussed in 10. The most common conventional strategy is plane-by-plane analysis based on the 2D defocus transfer function discussed in Section 6.4.3. Despite the challenges associated with the singularity in B(Dx, Dy, q), it is possible to estimate the 3D resolution that one might expect to obtain by direct inversion of Eqn. (6.73). As depicted in Fig. 6.15, for a circular aperture of diameter A the